Overview | steviol glycosides are structurally similar to steviol glycosides, with only one less glucose molecule at C13. Sweet tea glycoside is a non-toxic, high sweetness, low calorific value of sweet substances, with lowering blood pressure, lowering blood sugar, promoting metabolism, the role of the treatment of gastric acid, can be widely used in food, pharmaceutical industry, has been exported to Japan, Southeast Asia and other places. |
preparation | an extraction method of sweet tea glycosides, comprising the following steps: using 5g of sweet tea powder with a 60 mesh sieve, 15ml of 0.3% dodecyldimethylbetaine surfactant was added, and after cold soaking for 15min, the extraction was repeated twice with a solid-liquid ratio of 1:15,65 ° C., ultrasonic power of 200W for 35min. Suction filtration combined filtrate, rotary evaporator concentration to no alcohol taste. 60% ethanol overnight alcohol precipitation, 4000r/min centrifugation 20min, collect the supernatant of AB-8 resin, with 3BV 60% ethanol elution, eluate by D941 further decolorization deproteinization treatment, combine the column fluid and 5BV water wash solution, remove polyphenols, flavonoids and bitter substances by polyamide, finally collect the column fluid and rinse with water until no sweet taste, the column flow liquid and water washing liquid were combined and spray dried to obtain sweet white tea glycosides. The results showed that the content of sweet tea glycosides was 5.91% of the dry weight of sweet tea, and the extraction rate was 98.5%. |
detection method | CN201410705364.X reports a rapid detection method of sweet tea glycosides, the method comprises the following steps:(1) preparing the standard sample and the sample solution of the sweet tea glycoside respectively;(2) scanning the sweet tea glycoside standard sample at the wavelength of 190 ~ 400nm of the ultraviolet detector to obtain the best absorption wavelength;(3) the reversed-phase high performance liquid chromatography method was adopted. The HPLC conditions were as follows: Column: C18(5 μm,150 × 4.6mm I. d), the volume ratio of mobile phase composition: methanol: Water = 50~80:10~30, flow rate: 1.0/min, column temperature: 30 ℃, injection volume: 3~5 L;(4) to establish a standard curve, the content of sweet tea glycosides in the sample solution. |
biological activity | Rubusoside is a natural sweetener and solubilizer with excellent anti-angiogenic and anti-allergic properties. |
Use | rubusoside has the effects of lowering blood sugar, lowering blood pressure and promoting metabolism. |